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Figure 4 | Virology Journal

Figure 4

From: Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Figure 4

Western Blot analysis of the culture using H1N1 HA specific antibodies confirming the secreted expression of recombinant HA protein supernatants under denaturing and native conditions. Panel 4A: Under denaturing condition: Lane M: Pre-stained Protein marker (Fermentas, USA, #SM 0671) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 3: Cell culture supernatant from negative clone Panel 4B: Under native condition: Lane M: Pre-stained Protein marker (Fermentas, USA, #SM 0671) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 3: Cell culture supernatant from negative clone.

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Virology Journal

ISSN: 1743-422X