Design of peptides hbs-wt and hbs-kara. The consensus heparin binding motif in the DBP V3-like peptide of PvRII is contained in hbs-wt. The same alanine substitutions from a nonbinding mutant of PvRII (pv22KARA), tested in a previous study, were included in hbs-kara . Alanine-substituted amino acids are in blue and red. The N-terminus is to the left and is FITC-conjugated. The final residues at the C-terminus, DYKDDDDK, represent the FLAG epitope and are in bold.