Figure 4From: The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccineLuciferase expression in HEK293 cells. HEK293 cells were transfected for 48 h with the indicated plasmids containing the PCV-1 whole genome or Pcap DNA sequence inserted immediately upstream of either the SV40 promoter or CMV I/E promoter. Luciferase was quantified in cell lysates and each data point is the mean of 6 replicated transfections ± SD. * p < 0.01, **p < 0.05.Back to article page