Figure 3

(a-b). Houmal immunity responses by CD19⁺CD27⁺ and CD19⁺CD38⁺ B cells against polyprotein with different adjuvants measured by flow cytometry. Mice were immunized three times with the HCVE1+HVR1 polyprotein and splenocytes were isolated. The surface of cells stained for CD19(APC), CD27(PE) or CD38 (FITC). The data are presented as the mean percentage ± SD of CD19 positive cells of total CD27⁺ or CD38⁺ cells of spleens of four groups from four mice. The proportion of CD19⁺ CD27⁺ spleen lymphocyte of the four groups are 2.06 ± 0.34, 1.15 ± 0.08, 1.72 ± 0.49, 1.29 ± 0.78(a); The proportion of CD19⁺ CD38⁺ spleen lymphocyte of the four groups are 46.42 ± 2.21, 40.17 ± 6.40, 38.91 ± 11.52, 29.47 ± 8.49(b);