RNA interference-based resistance against a legume mastrevirus

Table 1 Quantitative real-time PCR estimation of viral DNA levels for CpCDPKV-inoculated transgenic and non-transgenic plants

Sample*	Mean Ct^@	Mean viral DNA concentration^@ (ng/10 ng genomic DNA)	Standard deviation of CT	Standard deviation viral DNA concentration
Standard 1	6.92	10	2.087	0.0000
Standard 2	12.61	1	0.107	0.0000
Standard 3	17.33	0.1	0.257	0.0000
Standard 4	21.1	0.01	0.877	0.0000
Standard 5	26.07	0.001	1.207	0.0000
Healthy 1	35	0.0000	0.0000	0.0000
Healthy 2	35	0.0000	0.0000	0.0000
Healthy 3	35	0.0000	0.0000	0.0000
Healthy 4	35	0.0000	0.0000	0.0000
Control 1	10.36	2.3	0.678	0.4632
Control 2	13.78	0.46	0.899	0.0927
Control 3	11.7	1.22	1.008	0.295
Control 4	10.74	1.92	0.256	0.467
Transgenic 1	26.85	0.0000938	0.7862	0.0000303
Transgenic 2	27.36	0.0000825	0.5655	0.00003065
Transgenic 3	27.07	0.0000842	0.5603	0.00005189
Transgenic 4	25.56	0.000172	1.453	0.00007732
Transgenic 5	25.73	0.000159	0.8234	0.00002111
Transgenic 6	25.83	0.000152	0.3278	0.00003265
Transgenic 7	26.5	0.000111	0.9562	0.00002111
Transgenic 8	26.77	0.0000972	0.4445	0.00002241
Transgenic 9	27.16	0.0000814	0.8129	0.00002231
Transgenic 10	27.35	0.0000813	1.421	0.00001317
NTC	35	0.0000	0.0000	0.0000

*The samples are indicated as non-inoculated healthy N. benthamiana plants (Healthy 1-4), infected non-transgenic plants (Control 1-4) and inoculated transgenic plants (Transgenic 1-10). The results of PCR reactions with known amounts of viral input (template) DNA, used to produce the standard curve, are indicated (Standard 1-5). A reaction with no input (template) DNA (NTC) was included as a control.
^@ For each sample PCR reactions were conducted in triplicate and the mean value and standard deviations between the values, for both the threshold cycle (Ct) and mean concentrations (in ng viral DNA per 10 ng of plant genomic DNA) are given.

ISSN: 1743-422X