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Table 1 Details of LAMP and PCR primers

From: Loop-mediated isothermal amplification for detection of porcine circovirus type 2

Primer namea

Genome positionb

Sequence (5'→3')

F3

1355-1378

CTCCAGTGCTGTTATTCTAGATGA

B3

1160-1181

TTTCCAGCAGTTTGTAGTCTCA

FIP

1273-1295

1317-1335

GAAGGGCTGGGTTATGGTATGGC-TTTTc-

ACAGCCCTCACCTATGACC

BIP

1240-1261

1182-1204

CCGCTACTTTACCCCCAAACCT-TTTTc-

GCCACAGCTGATTTCTTTTGTTG

P3

1721-1742

GCGGATCCGTGACGTATCCAAG

P4

1025-1049

CCACTAGTATAGGGGTTAAGTGGGG

  1. a F3, forward outer primer; B3, backward outer primer; FIP, forward inner primer; BIP, backward inner primer. b Genome position according to the JS2003 complete genome sequence (GenBank accession number AY578327). c Both inner primers of FIP and BIP containing 2 binding regions and connected by a TTTT bridge
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Virology Journal

ISSN: 1743-422X