Northern analysis of cytoplasmic and nuclear RNA fraction. RNA was extracted from uninfected BHK-21 cells (uninf.) and from cells infected with JEV at MOI of 0.1 at 48 h postinfection. Northern analysis was performed using DIG-labeled oligonucleotides complementary to nts 10,950-10,976 in the 3'-UTR (A) or to mitochondria RNA (mtRNA) (B). T, total RNA extract; C, cytoplasmic fraction, and N, nuclear faction.