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Table 3 Infectivity on Chenopodium quino a plants and number of infectious plasmids in plasmid pools obtained upon cloning by homologous recombination in yeast cells of ACLSV FL-cDNA under the control of the T7 promoter (T7-cDNA) or of the duplicated 35S promoter (70S-cDNA)

From: Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study

Plasmid pools

Infected/inoculated C. quinoa plantsa

Infectious plasmids per pool

T7-cDNA Pool 1

0/10

0

T7-cDNA Pool 2

0/26

0

T7-cDNA Pool 3

10/16

1

T7-cDNA Pool 4

0/26

0

70S-cDNA Pool 1

5/6

1

70S-cDNA Pool 2

4/6

1

70S-cDNA Pool 3

0/12

0

  1. a: 3 μg of capped transcripts per C. quinoa plant were used for inoculation of transcripts from T7-cDNA plasmid pools while about 160 ng of plasmids were used per plant for biolistic inoculation of 70S-cDNA pools (2 shots per plant).
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Virology Journal

ISSN: 1743-422X