Lycorine inhibited the replication of EV71 in muscle tissues of mice. The infected-mice were treated with placebo or lycorine at a dose of 0.4 mg/kg. The muscle tissues were sampled at 1 dpi, 3 dpi, 5 dpi and 9 dpi respectively (n = 8). A, the viral replication curve were determine by qRT-PCR (*: p < 0.05). B, the viral burden was confirmed by semi-quantitative RT-PCR.