Lycorine treatment reduced the mortality of EV71-infected mice. A, survival rates of the EV71 infected mice treated with placebo, ribavirin and lycorine (three different doses) were recorded to14 dpi (n = 40). *: p < 0.05, **: p < 0.001 (Survival rate of the mouse after 0.4 mg/kg lycorine treatment compared to that of the placebo). B, the clinical scores of infected-mice treated with placebo or lycorine (0.4 mg/kg) was systematically evaluated in an independent experiment (n = 40). *, p < 0.05. C, photos were a typical phenotype of ruffled hair and paralysis of hind limbs caused by EV71 infection at 8 dpi (left panel, indicated by arrow) and the phenotype was prevented by the lycorine treatment (right panel).