Lycorine inhibited the elongation of EV71 polyprotein. The RD cells were cultured in 96-well plate and infected with EV71 virus. The cells were treated with or without lycorine (1 μg/ml) at 6 hours post infection. A, the Viral RNA copies in RD cells were determined by qRT-PCR in a 0.5 hour intervals for 1.5 hours of treatment and the early stage of lycorine inhibition for virus was detected (*: p < 0.05); B, in the early stage of virus inhibition (0.5, 1.0 and 1.5 hours of lycorine treatment), the amounts of viral proteins in infected RD cells was detected by western blotting with the specific primary antibodies against different viral proteins. C, the intensity of bands of viral proteins in control cells were defined as 100, and the intensity of bands of viral proteins in lycorine-treated cells were compared respectively with those of saline-treated cells at 1 hour of treatment. These data are expressed as mean values of three independent experiments ± SD.