Figure 2
From: Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells
VOPBA analysis of HEV on swine liver cell membrane proteins. (A)The electroeluted protein was pre-incubated with virus particles before hybridization; (B) the transferred protein was directly incubated with the anit-HEV-ORF2 antibody without incubating with virus particles; (C) gel containing the swine liver cell membrane proteins was stained with Coomassie brilliant blue; (D) The PVDF membrane was incubated with virus particles and western blotting was then performed using a specific rabbit anit-HEV-ORF2 antibody, a single virus binding band about 55kDa was observed. Position of protein marker bands are shown in kDa