Figure 3

Identification of the HIF-1α domain responsible for the LTR activation. A. Schematic representation of the structural organization of the HIF-1α domains. B and C. 786-O cells were transfected with LTR-Luc along with HIF-1α full length and deletion mutant expression plasmids. The amount of DNA in each transfection mixture was normalized with pcDNA₆HisA. Luciferase activity was determined 48 hours after transfection. Results are displayed as histograms. Each transfection was performed 3 times using different DNA prep. D. Subcellular localization of HIF-1α. 786-O cells were transfected with c-myc-HIF-1α FL or deletion mutants as described in Methods section. Both proteins were detected in the nucleus and the cytoplasm (HIF-1α [1-531]) of the cells.