Figure 4

PCR-mediated amplification of the full-length genome of CLCuMV from inoculated Nicotiana plants. The ethidium bromide-stained agarose gel was photographed under UV illumination. The samples loaded on the gel resulted from PCR reactions with primer pair BegomoF/BegomoR and DNA extracted from the leaves of plants inoculated with CLCuMV (as indicated above each well). The samples in lanes C1 and C2 resulted from PCR reactions with DNA extracted from a healthy N. benthamiana plant and the plasmid containing the full-length genome of CLCuMV, respectively. Possible sub-genomic virus fragments are highlighted with white arrows. A DNA size marker was electrophoresed in lanes M. The sizes (bp) of selected marker bands are indicated on the left.