Figure 1From: The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirusThe specificity and sensitivity of one step SYBR green real-time RT-PCR. Plot of the amplification of a 10-fold serial dilution (1.31 × 107-1.31 × 102) of pDuCV-ORF-V1 to calculate the detection limit and sensitivity of real-time PCR by analyzing the fluorescence curve of the 191 bp DNA amplification product.Back to article page