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Figure 3 | Virology Journal

Figure 3

From: Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

Figure 3

Comparison of the sensitivity of the real-time and conventional PCR. A. The sensitivity of the real-time PCR assay with the 10-fold dilution series of the standard plasmid DNA from 5 × 107 to 5 × 100 copies. The X-axis represents cycles, and the Y-axis represents the fluorescence data. The detection limit was 50 copies. B. Electrophoresis analysis of conventional PCR with EF and ER primers. Lane 1: H2O (control). Lanes 2-9: Amplification results of 5 × 100 to 5 × 107 template copies. Lane 10: DNA Marker DL2 000.

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Virology Journal

ISSN: 1743-422X