Figure 1From: Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virusStandard curve for the real-time PCR. The X-axis represents copies of the plasmids, and the Y-axis represents the cycle threshold (C t). The assays were linear from 107 to 101 template copies; R2 was 0.998, and the reaction efficiency was 97% for the E gene.Back to article page