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Table 1 PCR primers used in NS5B-based in-house methods

From: Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C

Primer name Genotype Sequence Product Position Region NS5B*
NS5B2F All genotypes 5'-TTCACGGAGGCTATGACYAGG-3' 688 bp 1017-1038
NS5B-2R   5'-CGGGCATGMGACASGCTGTGA-3'   1704-1683
F56_1-3 1 and 3 5'-CACACTCCAGTYAAYTCCTGG-3' 241 bp 1204-1224
R56_1-3   5'-CWMCTGGAGAGTAACTGTGGAG-3'   1444-1423
GEN2FSN 2 5'-GACACTCCCCTGTCAATTCWTGG-3' 120 bp 1202-1224
GEN2RSN   5'-TGGTYCAGAGTGTCYTGGGC-3'   1321-1313
  1. *1a_.H77.NC_004102 - reference sequence that was used to calculate the positions of the NS5B region.
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