Table 1 PCR primers used in NS5B-based in-house methods
Primer name | Genotype | Sequence | Product | Position Region NS5B* |
|---|---|---|---|---|
NS5B2F | All genotypes | 5'-TTCACGGAGGCTATGACYAGG-3' | 688 bp | 1017-1038 |
NS5B-2R | 5'-CGGGCATGMGACASGCTGTGA-3' | 1704-1683 | ||
F56_1-3 | 1 and 3 | 5'-CACACTCCAGTYAAYTCCTGG-3' | 241 bp | 1204-1224 |
R56_1-3 | 5'-CWMCTGGAGAGTAACTGTGGAG-3' | 1444-1423 | ||
GEN2FSN | 2 | 5'-GACACTCCCCTGTCAATTCWTGG-3' | 120 bp | 1202-1224 |
GEN2RSN | 5'-TGGTYCAGAGTGTCYTGGGC-3' | 1321-1313 |