Figure 1
From: Gene Expression Profiles are Altered in Human Papillomavirus-16 E6 D25E-Expressing Cell Lines
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1743-422X-8-453/MediaObjects/12985_2011_Article_1568_Fig1_HTML.jpg)
Confirmation of HPV-16 E6 oncogenes expression and effects on the transcription of endogenous survivin gene in C33A cell lines with e6 oncogenes. A. Quantification of mRNA of wild-type E6 and E6 D25E in C33A cells by RT-PCR performed using OneStep RT-PCR kits. GAPDH mRNA was measured as a loading control. B. Protein expression analysis of wild-type E6 or E6 D25E using confocal microscopy. Caski cells were used as a positive control. C. Quantative real-time PCR specific for survivin gene.