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Figure 3 | Virology Journal

Figure 3

From: Expression of an IRF-3 fusion protein and mouse estrogen receptor, inhibits hepatitis C viral replication in RIG-I-deficient Huh 7.5 cells

Figure 3

Phosophorylation of STAT1 and STAT3 proteins in Huh7.5-IRF3ER cells. Huh 7.5 and Huh7.5-IRF3ER cells were used to detect phosophorylation of STAT1 and STAT3 proteins. Huh7.5-IRF3ER cells were treated without or with 4-HT to induce IRF-3ER dimerization. Total protein was extracted and analyzed by Western blotting with anti-p-STAT1 (S727) antibody, anti-STAT1 antibody (Figure A); anti-p-STAT3 (Y705) antibody and anti-STAT3 antibody (Figure B), respectively. Lane 1, Huh7.5 cells with 4-HT; lane 2, Huh7.5-IRF3ER without 4-HT; lane 3 to lane 9, Huh7.5-IRF3ER with 4-HT induction from 0.5 to 72 hours.

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Virology Journal

ISSN: 1743-422X