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Table 3 Primer pairs used for screening the suspected cases and for sequencing the complete genome of PCV2

From: Genetic characterization of Porcine Circovirus 2 found in Malaysia

Primer Sequence (5'-3') Size of PCR Product (bp) Nucleotide position PCR step Reference
PCV-1 forward CTC GGC AGC GTC AGT GAA AA 570 800-819 First step (xPCR) [39]
PCV-1 reverse AAA TTA CGG GCC CAC TGG CT   1350-1369   
PCV-2 forward CGG ATA TTG TAG TCC TGG TCG 481 1095-1115   
PCV-2 reverse ACT GTC AAG GCT ACC ACA GTC A   1549-1570   
PCV-1 forward CCT TCC GAG GAG GAG AAA AAC 491 879-900 Nested PCR (nPCR)  
PCV-1 reverse AAA TTA CGG GCC CAC TGG CT   1350-1369   
PCV-2 forward GGT TTG GGT GTG AAG TAA CGG G 329 1242-1263   
PCV-2 reverse ACT GTC AAG GCT ACC ACA GTC A   1549-1570   
CV1-forward AGG GCT GTG GCC TTT GTT AC 989 1336-1355 Sequencing [10, 23]
CV2-reverse TCT TCC AAT CAC GCT TCT GC   536-556   
CV3-forward TGG TGA CCG TTG CAG AGC AG 1093 453-471   
CV4-reverse TGG GCG GTG GAC ATG ATG AG   1525-1544