Genetic characterization of Porcine Circovirus 2 found in Malaysia

Table 3 Primer pairs used for screening the suspected cases and for sequencing the complete genome of PCV2

Primer	Sequence (5'-3')	Size of PCR Product (bp)	Nucleotide position	PCR step	Reference
PCV-1 forward	CTC GGC AGC GTC AGT GAA AA	570	800-819	First step (xPCR)	[39]
PCV-1 reverse	AAA TTA CGG GCC CAC TGG CT		1350-1369
PCV-2 forward	CGG ATA TTG TAG TCC TGG TCG	481	1095-1115
PCV-2 reverse	ACT GTC AAG GCT ACC ACA GTC A		1549-1570
PCV-1 forward	CCT TCC GAG GAG GAG AAA AAC	491	879-900	Nested PCR (nPCR)
PCV-1 reverse	AAA TTA CGG GCC CAC TGG CT		1350-1369
PCV-2 forward	GGT TTG GGT GTG AAG TAA CGG G	329	1242-1263
PCV-2 reverse	ACT GTC AAG GCT ACC ACA GTC A		1549-1570
CV1-forward	AGG GCT GTG GCC TTT GTT AC	989	1336-1355	Sequencing	[10, 23]
CV2-reverse	TCT TCC AAT CAC GCT TCT GC		536-556
CV3-forward	TGG TGA CCG TTG CAG AGC AG	1093	453-471
CV4-reverse	TGG GCG GTG GAC ATG ATG AG		1525-1544

ISSN: 1743-422X