Figure 3

Lectin staining in trachea, lung, and intestine from chickens. Examples of lectin staining demonstrating infAv receptors SNA lectin (SA-α-2,6-terminal saccharide) and MAAI and II lectin (SA-α-2,3-terminal saccharides), respectively, in trachea, lung, and intestine from chickens. The lectin stainings appear as a dark blue colour. A, B, and C demonstrate staining of the luminal surface of the tracheal epithelium by all three lectins SNA, MAAI, and MAAII, respectively, at the epithelium (arrowheads). The MAAI staining (C) is scarcer than the SNA (A) and MAAII (B) staining. D demonstrates SNA lectin staining of the lung of chicken, only a small area of positive staining of the epithelium in a parabronchus is seen (arrowhead). E demonstrates MAAI staining of the lung of a chicken, luminal staining of the epithelium in a parabronchus is seen (arrowhead). F demonstrates MAAII staining of the lung (arrowhead), the staining is less on the epithelium in the lumen of the lung compared to the MAAI staining (E). G, H, and I are intestinal epithelium demonstrating staining of the luminal surface of the epithelium (arrowheads) by all of the three lectins SNA, MAAI, and MAAII, respectively. (Original magnifications A, B, and C x40; D, E, F, G, H, and I x20).