Figure 1

SDS-PAGE Results of VP1 Protein. These results show that the structural protein VP1 after optimal expression has the size of 33 kDa, which is consistent with the expected size. 1: Uninduced control; 2: 3 hour induction; 3: Disintegrating liquid with ultrasonic; 4: Clarifying after disintegrating liquid with ultrasonic and centrifugation; 5: purifying sample solution with Ni Column, dissolved inclusion bodies; 6: Purifying flowing liquid with Ni Column; 7: Purifying Ni-Denature-8.0 wash solution with Ni Column; 8: Purifying Ni-Denature-6.0 wash solution with Ni Column; 9: Purifying Ni-Denature-4.0 equate with Ni Column;