Table 2 Primers used for RT-PCR and real-time PCR
Name | Forward primer (5'-3') | Reverse primer (5'-3') |
|---|---|---|
Prohibitin (RT-PCR, 835 bp) | GAAGATCTATGGCTGCCAAAGTGTTTGAG | CGGGATCCTCACTGGGGCAGCTGGA |
Prohibitin (real-time PCR, 129 bp) | AAACAGGTGGCTCAGCAGGAA | CAGTGAGTTGGCAATCAGCTCAG |
GAPDH (real-time PCR, 138 bp) | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |