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Figure 4 | Virology Journal

Figure 4

From: Characterization of the latent membrane protein 1 signaling complex of Epstein-Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation

Figure 4

Localization of LMP1 + TRAF BiFC. Cells were plated on coverslips and transfected with the indicated BiFC plasmids. Twenty-four hours post-transfection cells were fixed, counterstained with DAPI, and mounted. High resolution images were acquired using a confocal microscope. Individual colors, blue (DAPI), red (mCherry transfection control), green (BiFC), and merged images are displayed. Representative cells (white boxes) were magnified and various fluorescence localizations are indicated: perinuclear (white arrows), membrane (white arrowheads), and cytoplasmic (open arrowheads).

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