Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

Table 4 TAS-ELISA detection of T₄ transgenic and wild type plants inoculated with TMV/CMV, CMV/TMV or TMV+CMV.

Challenge virus^a	Plant lines	TMV		CMV
		I^b	N^b	I	N
	MP16-17-29-7	0/10^c (0.054)^d	0/10 (0.068)	10/10 (0.552)	10/10 (0.768)
TMV/CMV	MP16-17-29-9	0/10 (0.047)	0/10 (0.075)	10/10 (0.449)	10/10 (0.821)
	Wild plant	10/10 (0.778)	10/10 (0.829)	10/10 (0.578)	10/10 (0.813)
	MP16-17-29-7	0/10 (0.073)	0/10 (0.047)	10/10 (0.873)	10/10 (0.682)
CMV/TMV	MP16-17-29-9	0/10 (0.052)	0/10 (0.054)	10/10 (0.712)	10/10 (0.674)
	Wild plant	10/10 (0.852)	10/10 (0.852)	10/10 (0.748)	10/10 (0.652)
	MP16-17-29-7	0/10 (0.065)	0/10 (0.041)	10/10 (0.465)	10/10 (0.562)
TMV+CMV	MP16-17-29-9	0/10 (0.038)	0/10 (0.053)	10/10 (0.538)	10/10 (0.541)
	Wild plant	10/10 (0.754)	10/10 (0.882)	10/10 (0.564)	10/10 (0.518)

^aTMV/CMV represents plants were inoculated TMV firstly and then inoculated with CMV on new emergent leaves at 25 days after TMV inoculation. CMV/TMV represents plants were inoculated CMV firstly, and then inoculated TMV on new emergent leaves at 25 days after CMV inoculation. TMV+CMV represents plants were inoculated with TMV and CMV at the same time.
^bI represents inoculated leaves, N represents new emergent leaves
^cNumber of infected plants/number of inoculated plants.
^dNumber in brackets was average absorbance values of three independent ELISA assay.

ISSN: 1743-422X