Skip to main content

Table 2 Segregation of TMV or CMV resistance over the T2, T3 and T4 generations of Nicotiana tabacum transformed with inverted repeats of the partial TMV movement protein (MP) gene or the partial CMV Replication protein (Rep) gene.

From: Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

T1 line

No. of T2plants showing R/Sa

T2line

 

No. of T3plants showing R/S

Responses of T4progenies

 

R

S

  

R

S

 

MP16-17

45

5

MP16-17

-3

50

0

All immune

    

-29

50

0

All immune

    

-58

50

0

All immune

MP31-28

36

14

MP31-28

-31

41

9

Variable (47/3)c

    

-43

37

13

--d

    

-55

40

10

--

MP39-36

39

11

MP39-36

-25

33

17

Variable (42/8)

    

-37

39

11

--

    

-49

38

12

--

MP53-52

43

7

MP53-52

-7

42

8

Variable (46/4)

    

-21

45

5

Variable (48/2)

    

-46

44

6

Variable (48/2)

MP36-17

0

50

MP36-17

-2

0

50

All susceptible

Wt+b

0

50

Wt+

 

0

50

All susceptible

Rep15-1

50

0

Rep15-1

-1

50

0

All immune

    

-7

50

0

All immune

    

-32

50

0

All immune

Rep15-30

46

4

Rep15-30

-7

39

11

Variable (42/8)

    

-23

46

4

Variable (45/5)

    

-38

42

8

Variable (48/2)

Rep15-66

36

14

Rep15-66

-7

32

18

Variable (40/10)

    

-36

37

13

Variable (41/9)

    

-48

35

5

Variable (38/12)

Rep17-8

21

29

Rep17-8

-15

22

28

--

    

-24

0

50

All susceptible

Rep727-1

0

50

Rep727-1

-20

0

50

All susceptible

Wt+b

0

50

Wt+

 

0

50

All susceptible

  1. aR indicated immune and S indicated susceptible with clear mosaic symptoms.
  2. bWt+ represented wild type Nicotiana tabacum inoculated with TMV or CMV.
  3. cImmune/susceptible.
  4. d--Not tested.