Table 2 ChiP primers
Encompassing the KLF6 binding site at the TGF-β promoter | TGF-β forward 5' -AAGGAGGCTGGGTTGGAAACTC-3' TGF-β reverse ' 5 -TGGGACCACACCTGGAAATG-3' | Product: 181 bp (encompassing -85 to -265 region of TGF-β promoter) | Optimal annealing temp: 58°C. |
|---|---|---|---|
Encompassing the region in TGF-β promoter that does not bind to KLF6 (negative control) | TGF-β forward 5' -CCTCTTTCTCTGGTGACCCAC-3' TGF-β reverse ' 5 -CCACCGTCCTCATCTCGC-3' | Product: 185 bp (encompassing -1028 to -1212 region of TGF-β promoter) | Optimal annealing temp: 60.6°C. |