Skip to main content

Table 1 List of artificial templates containing wild type and NA-resistance mutations.

From: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example

Oligo name

Oligo Sequence

Location in reference strains

Description

N1 274H

95nt

5'TCGTACAAAATCTTCAAGATCGAAAAGGGAAAGGTTACTAAATCAATAGAGTTGAATGCACCCAATTTTCATTATGAGGAATGTTCCTGTTACCC 3'

H1N1 (CY12306.1, 763-857)

Wild Type

N1 274Y

95nt

5'TCGTACAAAATCTTCAAGATCGAAAAGGGAAAGGTTACTAAATCAATAGAGTTGAATGCACCCAATTTTTATTATGAGGAATGTTCCTGTTACCC 3'

H1N1 (CY12306.1, 763-857)

Resistance mutation

N1 274R

96nt

5'TTGATTAAAAGACACCCAAGGTCGATTTGAACCATGCCAGTTGTCCCTGCATACACACATCACTGTGCCAGTGTCTGGGTAACAGGAACATTCCTC3'

H1N1 (CY12306.1, 933-837)

Antisense oligo

H5N1 274H

95nt

5'TCACATAAGATCTTCAAAATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCC 3'

H5N1 (DQ493076, 694-788)

Wild Type

H5N1 274Y

95nt

5'TCACATAAGATCTTCAAAATGGAAAAAGGGAAAGTGGTTAAATCAGTCGAATTGGATGCTCCTAATTATTACTATGAGGAATGCTCCTGTTATCC 3'

H5N1 (DQ250165,

694-788)

Resistance mutation

H5N1 274R

96nt

5'TTGATTGAAAGATACCCATGGCCGATTTGAGCCATGCCAATTATCCCTGCACACACATGTGATTTCGCCGGCATCAGGATAACAGGAGCATTCCTC 3'

H5N1 (DQ250165,

864-768)

Antisense oligo

N2-119GLU

90nt

5'TCTAAGGACAATTCGATTCGGCTTTCCGCTGGTGGGGACATCTGGGTGACAAGAGARCCTTATGTGTCATGCGATCCTGACAAGTGTTATC 3'

H3N2 (CY016653,305-395)

Wild Type

N2-119Val

90nt

5'TCTAAGGACAATTCGATTCGGCTTTCCGCTGGTGGGGACATCTGGGTGACAAGAGTNCCTTATGTGTCATGCGATCCTGACAAGTGTTATC 3'

H3N2 (CY016653,305-395)

Resistance mutation

N2-119GLY

90nt

5'TCTAAGGACAATTCGATTCGGCTTTCCGCTGGTGGGGACATCTGGGTGACAAGAGGNCCTTATGTGTCATGCGATCCTGACAAGTGTTATC 3'

H3N2 (CY016653,

305-395)

Polymorphisms

N2-119ALA

90nt

5'TCTAAGGACAATTCGATTCGGCTTTCCGCTGGTGGGGACATCTGGGTGACAAGAGCNCCTTATGTGTCATGCGATCCTGACAAGTGTTATC 3'

H3N2 (CY016653,305-395)

Polymorphisms

N2-119ASP

90nt

5'TCTAAGGACAATTCGATTCGGCTTTCCGCTGGTGGGGACATCTGGGTGACAAGAGAYCCTTATGTGTCATGCGATCCTGACAAGTGTTATC 3'

H3N2 (CY016653,

305-395)

Polymorphisms

N2-119R

98nt

5'CCGATAAGGGGTCCTATCATGTACTGTGTCATTTGAATGCCCGTTGTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGATCGC 3'

H3N2 (CY016653,

472-375)

Antisense oligo

N2-292ARG

89nt

CAGCATGTCGAGGAGTGCTCCTGTTATCCTCGATATCCTGGTGTCAGATGTGTCTGCAGRGACAACTGGAAAGGCTCCAATAGGCCCAT3'

H3N2 (CY016653,

821-909

Wild Type

N2-292LYS

89nt

5'CAGCATGTCGAGGAGTGCTCCTGTTATCCTCGATATCCTGGTGTCAGATGTGTCTGCAARGACAACTGGAAAGGCTCCAATAGGCCCAT 3'

H3N2 (CY016653,

821-909

Resistance mutation

N2-292R

99nt

5'TTTCTGGGTGTGTCTCCAACAAGTCCTGAGCACACATAACTGGAAACAATGCTATAATCCTTTACATTTATATCTACGATGGGCCTATTGGAGCCTTTC 3'

H3N2 (CY016653,

987-889

Antisense oligo

  1. 1: Synthetic templates containing the wild type, naturally occurring polymorphisms and resistance mutations. These genome locations were indicated in the table according to reference strains.
  2. 2: Antisense oligo (R) with complementary sequences at 3' end were also synthesised and the complementary sequence were shown as underline.
Back to article page

Virology Journal

ISSN: 1743-422X