Figure 3
From: Latent HIV in primary T lymphocytes is unresponsive to histone deacetylase inhibitors
VPA or TSA still remained ineffective on high percentages of latent HIVs in primary T cells. Panel A, High percentages of latently infected cells were generated on H80 as described in the text and treated with with 500 nM prostratin (B), 2 mM VPA (C) and 150 nM TSA (D) for 18 hours. The percent increases in p24+ cells were determined by intracellular staining and flow cytometry. Untreated control (Mock) was shown in panel A. Panel E shows the relative changes in HIV-gag RNA levels after various treatments. The relative levels of intracellular HIV-gag RNAs were measured by real time quantitative RT-PCR (see text). Prostratin could induce the levels of HIV-gag RNA by 1.7-fold (p < 0.01) in primary cells, compared to untreated (mock) control, whereas VPA did not induce the level of gag-RNA in these cells. The numbers on top of each bar indicate fold change relative to untreated (mock) control. Results shown here are from triplicate samples in a representative experiment. Error bars indicate standard deviations. The statistically significant data (p < 0.01) determined by Student's t-test is indicated by an asterisk.