Figure 7
From: The matrix (M) protein of newcastle disease virus binds to human bax through its BH3 domain
Subcellular localization of Bax in pEGFP-M-transfected HeLa cells. At 20 h post-transfection the protein samples were analyzed by Western blotting analysis with the anti-Bax 2D2 antibody. Transfected adherent cells were collected separately from transfected floating cells (c = cytosolic fraction, m = heavy membrane fraction, u = untransfected cells, a = transfected adherent cells, f = transfected floating cells). Bax translocated to the heavy membrane fraction after transfection with pEGFP-M, especially in transfected floating cells.