Figure 6
From: The matrix (M) protein of newcastle disease virus binds to human bax through its BH3 domain
Transient transfection of HeLa cells with pEGFP-M leads to apoptosis. A. HeLa cells were transfected with either pEGFP or pEGFP-M construct. At 20 h post-transfection, cells were stained with DAPI and visualized by fluorescence microscopy. Arrow indicates a dead EGFP-M-positive cells. Arrow-head indicates a dead cell that may have lost its fluorescence due to possible degradation of EGFP-M protein. B. Percentage of condensed nuclei as compared to control. C. Percentage of EGFP-positive cells. Each data point represents three independent measurements, and the error bars indicate the standard error of the mean.