Figure 4
From: Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae
Agarose gel electrophoresis of the cDNA obtained by the single-primer amplification technique. (A) Lane St, MassRuler™ DNA Ladder 80-10,000 bp (Fermentas); lanes 1 and 2, PCR fragment corresponding to cDNA obtained from 2.2 kbp dsRNA from B. cinerea CCg378. The numbers on the left side indicate molecular sizes expressed in kilobase pairs (kbp). (B) Analysis of the digestion patterns with restriction enzymes of the recombinant plasmid (pGEM-T easy + cDNA) containing as insert the cDNA shown in (A). Lane St, Lambda DNA/Eco RI + Hin dIII marker; lane 1, recombinant plasmid treated with Nco I; lane 2, recombinant plasmid treated with Nco I and Spe I; lane 3, recombinant plasmid without treatment. The numbers on the left side indicate molecular sizes expressed in kilobase pairs (kbp).