Figure 1
From: Evolutionary variation of papillomavirus E2 protein and E2 binding sites
Consensus Sequence Analysis of E2BSs Throughout Papillomavirus Genera. Well characterized E2BSs from HPV16, 18, and BPV1 were analyzed using MEME software to generate a consensus E2BS motif (a). This motif was then utilized by MAST software to search through the full-length genomes of 128 papillomaviruses obtained from NCBI to identify sequences with high-identity to the consensus. The average number of E2BSs identified per genome was sorted into the six largest papillomavirus genera and were further analyzed to determine if the binding sites were located inside or outside the LCR of the genomes (b). Identified E2BSs were then manually analyzed to determine the GC content of their four base spacer regions. Results were again calculated in terms of average GC content of E2BSs for each of the individual papillomavirus genera both inside and outside the LCR as well as in total (c). Finally, the identified binding sites were used for MEME analysis to identify the consensus E2BS motif for each of the six papillomavirus genera analyzed in this study (d).