Figure 6

Early alterations of cytoskeletal proteins in UL14D-infected cells. HEp-2 cells were infected with wild-type and UL14D virus at an MOI of 3. Cells were fixed at the indicated time points, permeabilized and stained with Alexa Fluor^®546-conjugated phalloidin and anti-α tubulin (diluted 1:1000). Following infection with UL14R and UL14D, actin stress fibers and the microtubule mesh became reduced from 7 h.p.i. in UL14D-VP16GFP-infected cells (H, I). At 17 h.p.i. actin stress fibers remained in UL14R (K), whereas UL14D-infected cells had no stress fibers and exhibited formation of cell-to-cell filamentous processes of actin (N).