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Figure 5 | Virology Journal

Figure 5

From: Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16

Figure 5

Intracellular localization of viral non-structural or tegument proteins. (A-H) Comparison of localization among VP16GFP, ICP8, and UL34 in UL14R- or UL14D-VP16GFP-infected cells. HEp-2 cells were infected at an MOI of 3, fixed at 7 and 17 h.p.i., permeabilized, and treated with anti-ICP 8 and anti-UL34 rabbit polyclonal antisera (diluted 1:1000) [21]. The structures of the replication compartments represented by ICP8 did not differ between cells infected with UL14R-VP16GFP (B, F) and UL14D-VP16GFPF (D, H) at the two time points. The inner nuclear membrane represented by UL34 staining revealed an apparent dent in the nucleus only in UL14D-infected cells, and this was accompanied by the formation of the VP16GFP aggregates (H).

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Virology Journal

ISSN: 1743-422X