Figure 3

Intracellular localization of VP16GFP in cells infected with UL14-mutant viruses. HEp-2 cells were infected with the virus at a MOI of 3 PFU/cell with the following viruses: (A-C) UL14R-VP16GFP, (D-F) UL14D-VP16GFP, (G-I) UL14(K51M)-VP16GFP, (J-L) UL14(3P)-VP16GFP, (M-O) UL14(3P)R-VP16GFP. The cells were then fixed at 7, 11, and 17 h.p.i. and examined using laser confocal microscopy (Zeiss LSM510). UL14D-VP16GFP infection induced prominent cell rounding and the accumulation of VP16GFP at the periphery of the nucleus (E, F). This phenotype, although less distinct, was observed in cells infected with UL14(3P)-VP16GFP, especially at later time points after infection (K, L). Open arrows indicate clumps of VP16GFP that were readily observed (H, K). All images were acquired with the same magnification. Scale bars: 10 μM.