Figure 1

Generation of UL14 mutant viruses. (A) Comparison of the amino acid (aa) sequences of UL14 (residues 48-108) of simplex type herpesviruses (human herpesvirus type 1 (HHV1), human herpesvirus type 2 (HHV2), herpesvirus B virus (HeBV), and Saimiriine herpesvirus 1 (SaHV)) and Hsp70 (residues 497-555) of human Hsp70 (HSP70_Hu), Rat Hsp70 (HSP70_Ra), and mouse Hsp70 (HSP70_Mo). The letters shown in black are identical to those of HHV-1 UL14, and the letters shown in gray are similar residues. The star indicates residue K51. The asterisks indicate three residues that are substituted in UL14(3P). (B) A schematic diagram of the UL14 wild-type (219 aa) and amino acid-substituted mutant proteins. The gray box indicates the HSP-like homology domain of UL14 (aa 48-108), and the thin black bars show the substituted residues introduced in this study.