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Table 1 Primers and probe used in real-time PCR assay for Pbo-likeV

From: Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV)

Gene

Primer-probe

Sequence

Positiona

NP1

Forward

5'-TCGAGCTATACAACCGAAGAAGAGA-3'

37-61

 

Reverse

5'-TGTTTCGGAGATGTCCTTGCT-3'

93-113

 

Probe

FAM-5'-CAGCTCTTCGAATCGCCGCTCTCC-3'-TAMRA

63-86

  1. aNucleotide position was designated according to NP1 gene of Pbo-likeV strain JSNJ1 (GenBank accession no. HQ872052)
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Virology Journal

ISSN: 1743-422X