Figure 5
From: Infectious alphavirus production from a simple plasmid transfection
Stability of Reporter Gene Expression. BHK or C636 cell lines were infected with P0 derived pBG452 virus at MOI = 0.01 for 48 hrs, then media was collected and titered. This process was repeated for a total of 3 passages. After the 3rd passage, BHK or C6/36-derived virus from each passage was added to BHK or C6/36 cells at MOI = 0.01 and incubated for 28 (BHK) or 86 (C6/36) hrs. mCherry fluorescence was detected at the indicated times by fluorescence microscopy.