Figure 5
From: Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells
Purification of secreted, recombinant CHIKV E1ΔTM and E2ΔTM proteins. Sf 21 cells were infected with Ac-6KE1ΔTM and Ac-E3E2ΔTM and the respective serum-free medium fractions were loaded onto Talon® spin columns. Bound proteins were eluted 3 times with 150 mM imidazol. The elution fractions were analyzed with CBB (A) and western blotting, using α-E1 and α-E2 polyclonal antisera (B). NP represents non-purified fractions and El.1-3 indicate the subsequent elution fractions. Total protein concentration of the elution fractions (1 ml each) are noted above in μg/ml.