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Figure 2 | Virology Journal

Figure 2

From: Single amino acid change in gp41 region of HIV-1 alters bystander apoptosis and CD4 decline in humanized mice

Figure 2

V38E mutant, compared to WT virus, is limited in inducing CD4 cell decline in the presence of similar levels of virus replication. Hu-HSC mice were infected with 50,000 TCID50 of viruses and bled every 2 weeks for a total of 8 weeks and then sacrificed. (A) CD4 and CD8 levels in PBMCs from the mice were determined after staining with CD45, CD4 and CD8 antibodies. A representative histogram of PBMC obtained at 8 weeks postinfection (top) and cumulative data on serial CD4 counts (bottom) is shown. (B) Splenocytes collected at 8 weeks postinfection were assayed for CD4 and CD8 levels as above. A representative histogram (top) and cumulative data (bottom) is shown. (C) Plasma collected at the indicated time points postinfection were assayed for vriemia using p24 Ag capture ELISA. n = 3 mice per group. (* p < 0.05. ** p < 0.001)

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Virology Journal

ISSN: 1743-422X