BVK168 passage | Titer (Pfu/100 μl) | VSV | VACV | CPXV | SINV | PIXV | INKV | USUV |
|---|
1st | BVK168 | 4.5 × 106 | 7 × 106 | 4.9 × 105 | 2 × 107 | 1 × 108 | 7 × 105 | 1.6 × 103 |
| | Original line* | 4.5 × 105 | 1 × 106 | 7 × 105 | 2 × 107 | 1 × 108 | 1 × 105 | 1 × 105 |
2nd | BVK168 | 2 × 106 | 6.5 × 106 | 2.64 × 106 | n.d. | n.d. | 7.2 × 105 | 8.2 × 103 |
| | Original line* | 4.8 × 105 | 3 × 106 | 1.4 × 106 | n.d. | n.d. | 2.2 × 105 | 1 × 105 |
- * original cell lines are shown in table 1
- Abbreviations: p.i., post infectionem; Pfu, plaque forming units; VSV, vesicular stomatitis virus strain India; VACV, vaccinia virus strain Munich 1; CPXV, cowpox virus strain 81/01; SINV, Sindbis virus strain C377; PIXV, Pixuna virus strain BeAr35645; INKV, Inkoo virus strain TN-98-5085; USUV; Usutu virus strain 0679/2006; cf, culture frozen until further use; n.d., not determined
- Titers gained on BVK168 cells in comparison to back-titration on original cell line. Puumalavirus and Borna disease virus are not listed as these showed no visible CPE and evidence for infection of cells by these viruses had to be confirmed by real-time RT-PCR and/or immunofluorescence assay.
