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Figure 2 | Virology Journal

Figure 2

From: A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Figure 2

Efficient replication of viruses containing K65R+L74I mutations in MT-2 cells. In order to understand the replication of mutant viruses in cells containing higher dNTP pools, 3 × 106 MT-2 cells (0.5 × 106/ml) were infected with 0.001 MOI of 293 cells-derived viruses. Culture supernatants were collected at various time points and RT activity was determined. The graph shows a more profound difference in replication kinetics of K65R+L74I versus K65R+L74V viruses in MT-2 cells in comparison to that observed in PBM cells.

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Virology Journal

ISSN: 1743-422X