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Figure 1 | Virology Journal

Figure 1

From: A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Figure 1

L→I but not L→V change at RT codon 74 results in a replication competent virus in the background of K65R mutation. PHA-stimulated PBM cells (10 × 106) were infected with 293T-derived viruses containing MOIs: 0.001 (A), 0.01 (B), 0.1 (C) and 0.01(D) and culture supernatants were collected at various time points. RT activity (A, B, and C) and antigen p24 (D) was determined to monitor viral replication. The plot shows efficient replication with a sigmoid growth curve for K65R+L74I virus suggesting the yield of a replication competent virus. Viruses with K65R+L74V mutant virus did not show measurable RT activity until day 14 at 0.001 MOI. At higher MOIs (0.01 and 0.1), measurable RT activity (B and C) or antigen p24 (D) was observed after day 10 in viruses with K65R+L74V mutation.

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Virology Journal

ISSN: 1743-422X