Figure 5

NIM811 blocks the release of HCV RNA and infectious virions from infected cells. Huh7.5 cells were infected with JFH1 HCVcc for three days. Following infection, cells were washed and treated with the indicated compounds for 8h. Intracellular and extracellular virus was harvested as described in materials and methods, and assayed for viral RNA content by qRT-PCR (A and B) or infectivity (C and D). GolgiPlug prevents secretion of infectious particles, resulting in an accumulation of intracellular viral RNA and infectious particles. There is a dose dependent decrease in viral RNA and infectious particles in the media of infected cells treated with NIM811.