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Figure 4 | Virology Journal

Figure 4

From: Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: Case Report

Figure 4

Serum cytokine levels analyzed by multiplex Flow Cytometry. Serum cytokine levels were analyzed with a BenderMed Systems Human 11-Plex Inflammatory Cytokine kit, and an Accuri C6 Flow Cytometer equipped with dual laser detection. Data was processed and quantitated with Flow Cytomic Pro software, and plotted on linear scales. G-1180 presented with elevated levels of IL-12p70, IL-6, and IL-10, all of which decreased by the following day. On day 10 post onset of disease a significant but transient spike in IL-1β, IL-6, IL-8, TNF-α, and IL-10 occurred, that rapidly decreased overnight, mostly to background levels. The only notable cytokine fluctuation in G-1180 contacts occurred in -A.2 with a dramatic spike in IL-8 that decreased to background levels within 2 days (-A.3). The single point analysis for G-1177 revealed high levels of IL-1β, IL12p70, IL-6, and IL-10.

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Virology Journal

ISSN: 1743-422X