Figure 1
From: Isolation and characterization of a novel alphanodavirus
Electron microscopic study of HzNV. (A) Co-existence of HzNV and Hear NPV in Hz-AM1 cells. Fresh Hz-AM1 cells grown in monolayer were infected with H. armigera hemolymph bearing recombinant Hear NPV. Cells were processed for TEM at 2 dpi. N: nuclear; C: cytoplasm; NC: nuclear capsid; ph: polyhedra. (B) Crystal-arrayed HzNV in the cytoplasm of Hz-AM1. Hz-AM1 cells were infected with purified HzNV and processed for TEM at 3 dpi. (C) Negative staining of purified HzNV virions. (D) Membrane structures were joint with crystal array of HzNV in the cytoplasm of HzNV infected Hz-AM1 cell. Hz-AM1 cells were infected with HzNV. At 2 dpi, cells were processed for TEM. The vacuole indicated by a solid arrow displayed some residual membrane structures, while the vacuole indicated by the open arrow was filled with HzNV particles with no structure. N, nuclear, NM, nuclear membrane.