Figure 5

Global comparison of antibody binding levels of human plasma from 16 different donors against 16 different influenza T-B and B-T peptides, HIV gag peptide (HIV) and recombinant A/New Caledonia/20/99 hemagglutinin protein (rHA NC) as measured by ELISA. Absorbance values (OD 450 nm) are given as a mean of duplicates at a 1/100 dilution of plasma with the subtraction of non-specific binding levels (absorbance value of plasma alone control wells) ± SEM. Individual absorbance values (as presented in Figure 4) are shown by black circles, while mean OD values (average of all donors) are represented by a bar. The assay was run on three separate days and data shown is representative of all results. All peptides except 128, 129, 132, 135, and 136 had a p-value lower than 0.05 in a Mann Whitney test.