Figure 4

Phylogenetic relationship of Sudan-Lagawa strains of CCHFV. Partial S segment sequences of the virus strains were aligned with other CCHFV strains from Sudan, different African regions, the Middle and Far East, Asia, and Eastern Europe. Sequeces were analyzed with the Lasergene 3.57 software package (DNASTAR, Madison, WI, USA). Phylogenetic tree was constructed using PAUP version 4.0 (Sinauer Associates, Inc., Sunderland, MA, USA). Bootstrap values were calculated from analysis of 500 replicates of the data set, and values greater than 50% are indicated at the appropriate nodes. Each CCHFV sequence is designated by the name of the strain, country of origin. The GenBank accession numbers are given for each CCHFV isolate. The two Lagawa strains from Sudan (Sudan Lagawa1 and 2-2010) are highlighted in red color for clarity (GenBank accession number (HG829854). Brace indicates group III virus genetic lineage (16).